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Decrease in platelet reduced glutathione increases lipoxygenase activity and decreases vitamin E
Author(s) -
Calzada Catherine,
Véricel Evelyne,
Lagarde Michel
Publication year - 1991
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535616
Subject(s) - chemistry , malondialdehyde , lipoxygenase , glutathione , lipid peroxidation , arachidonic acid , antioxidant , platelet , biochemistry , glutathione peroxidase , vitamin e , thiol , high performance liquid chromatography , clinical chemistry , chromatography , medicine , enzyme
Unstimulated normal human blood platelets were treated with azodicarboxylic acid bis (dimethylamide) (diamide), a thiol‐oxidizing agent. Oxygenated arachidonic acid (AA) metabolites, malondialdehyde (MDA), and tocopherols were then quantified by high‐performance liquid chromatography (HPLC). Diamide treatment partially decreased the amount of reduced glutathione (GSH) content and induced a subsequent decrease in peroxidase activity. However, formation of 12‐hydroxy‐eicosatetraenoic acid (12‐HETE), the end‐product of lipoxygenation of AA, increased. Formation of MDA, a marker of overall lipid peroxidation, was also enhanced. Furthermore, platelet α‐tocopherol, but not γ‐tocopherol, significantly decreased. These results indicate that enhanced “basal” lipoxygenase activity, as a marker of specific AA oxygenation, may be linked to decreased platelet antioxidant status.