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Evidence for loss of tritium from 3β‐tritiated deoxycholic acid during enterohepatic cycling in man
Author(s) -
Marcus S. N.,
Heaton K. W.
Publication year - 1988
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535611
Subject(s) - deoxycholic acid , chemistry , tritium , bile acid , enterohepatic circulation , metabolism , chromatography , medicine , radiochemistry , biochemistry , physics , nuclear physics
Abstract A double‐label single‐intubation isotope technique was assessed for studying deoxycholic acid metabolism by simultaneous IV administration of [3β‐ 3 H]deoxycholic acid and [24‐ 14 C]deoxycholic acid to five healthy subjects. The 3 H/ 14 C ratio in duodenal bile fell progressively in all subjects, the mean reduction on day four being 48.8%. When the same labeled bile acids were administered to three ileostomy subjects, again the ratio fell with a mean reduction of 44.4% on day four. This fall more likely was due to hepatic exchange of tritium for hydrogen, rather than to colonic bacterial oxidation. [3β‐ 3 H]Deoxycholic acid should not be used to study the kinetics and metabolism of deoxycholic acid.