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Antitumor activity of synthetic alkylphospholipids with or without PAF activity
Author(s) -
Kudo Ichiro,
Nojima Shoshichi,
Chang Hyeun Wook,
Yanoshita Ryohei,
Hayashi Hidetoshi,
Kondo Eri,
Nomura Hiroaki,
Inoue Keizo
Publication year - 1987
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535545
Subject(s) - lipidology , clinical chemistry , chemistry , pharmacology , medicine , biochemistry
1‐ O ‐Octadecyl‐2‐ O ‐methyl‐ sn ‐glycero‐3‐phosphocholine (ET‐18‐OMe) has been reported to possess definite antitumor activity in vivo. Twenty‐two alkyl lysophospholipid analogs were chemically synthesized, and their antitumor activity against mouse experimental tumors (Sarcoma 180, MM46, P388) was examined. Among them, 1‐ O ‐octadecyl‐2‐ O ‐acetoacetyl‐ rac ‐glycerol‐3‐phosphocholine was found to show antitumor activity similar to ET‐18‐OMe with less acute toxicity. Intravenous injection of the ET‐18‐OMe with sn ‐3 configuration retarded the subcutaneous growth of Sarcoma 180 cells effectively, while the growth inhibition by the sn ‐1 isomer was much less effective. This stereospecificity was similar to that observed in their activities as platelet‐activating factor (PAF) agonists. The acetoacetyl compound, another PAF agonist, showed similar stereospecific antitumor action in vivo. These findings suggest that some alkyl lysophospholipids may activate host cells to a cytostatic stage against tumor cells in vivo through binding to a PAF receptor. Our preliminary results indicated that the responsible cells under these conditions might be primarily immature macrophages present in the bone marrow. No appreciable or even adverse stereospecificity was observed in the different sets of experiments where the activity of ET‐18‐OMe against MM46 tumor cells in vivo or the direct cytotoxicity against human promyelocytic leukemia HL‐60 cells in vitro was examined. Under, some conditions, the antitumor activity of ET‐18‐OMe in vivo may be revealed through direct cytotoxicity and/or modulation of the host defense system by “nonspecific” mechanisms. Some alkylphospholipids without PAF activity may also show antitumor activity through similar, “nonspecific” mechanisms.