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Effect of lipid derivatives on invasion in vitro and on surface glycoproteins of three rodent cell types
Author(s) -
Storme Guy A.,
Bruyneel Erik A.,
Schallier Denis C.,
Bolscher Jan G.,
Berdel Wolfgang E.,
Mareel Marc M.
Publication year - 1987
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535542
Subject(s) - in vitro , phospholipid , chemistry , cell , transfection , microbiology and biotechnology , biochemistry , cytotoxicity , cell type , rodent , cell culture , biology , gene , membrane , ecology , genetics
The antiinvasive activity on MO 4 mouse cells of the following lipid derivatives was tested in vitro: an alkyllysophospholipid derivative (BM 41.440), an alkyl‐linked lipoidal amine (CP‐46,665) and a naturally occurring ester‐linked phospholipid (2‐LPC). In this test, BM 41.440 had the same antiinvasive potency as ET‐18‐OCH 3 , whereas CP‐46,665 and 2‐LPC had no effect on invasion. Comparison of the antiinvasive effect of ET‐18‐OCH 3 on three types of cells showed the following ranking: 12R1C‐RK rat kidney adenovirus type 12 transfected cells>MO 4 mouse cells>LLC‐H61 Lewis lung carcinoma cells. This ranking was not reflected in ET‐18‐OCH 3 ‐induced changes of cell surface exposed glycopeptides derived from the three types of cells metabolically labeled with radioactive fucose. The present and previous experiments suggested that changes in invasion caused by lipid derivatives depended upon relative cell surface fucosylglycopeptide alterations in both the invasive cells and the normal tissue.