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Nanogram quantification of nonpolar lipid classes in environmental samples by high performance thin layer chromatography
Author(s) -
Conte Maureen H.,
Bishop James K. B.
Publication year - 1988
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535526
Subject(s) - degree of unsaturation , derivatization , chromatography , chemistry , thin layer chromatography , high performance thin layer chromatography , composition (language) , coefficient of variation , carbon number , analytical chemistry (journal) , high performance liquid chromatography , organic chemistry , linguistics , philosophy , alkyl
A sensitive, simple, and rapid high performance thin layer chromatography (HPTLC) method that quantifies nanogram amounts of complex nonpolar lipid classes in environmental samples with a minimum of sample preparation is presented. The derivatization method is lipid‐specific and insensitive to carbon number or the degree of unsaturation of the fatty acids composing the lipid class compounds. Nonpolar lipid classes ranging from 10 to over 500 ng easily may be quantified in the same sample run. The coefficient of variation for sample replicates on different plates ranged from 2.3% to 5.9%. Accuracy of the method is better than 15%. The nonpolar lipid class composition of <53 μm oceanic particles in a vertical depth profile of the upper 600 meters of the Slope Water was determined to illustrate the HPTLC method. The observed changes in particulate lipid class composition indicate that secondary production by deep‐living organisms significantly alters suspended particle composition with depth in the water column.