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Disposition and metabolism of pristane in rat
Author(s) -
Le Bon A. M.,
Cravedi J. P.,
Tulliez J. E.
Publication year - 1988
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535514
Subject(s) - pristane , chemistry , metabolism , hydroxylation , chromatography , phosphatidylserine , urine , excretion , metabolite , biochemistry , phospholipid , hydrocarbon , organic chemistry , enzyme , membrane
Abstract The fate of pristane (2,6,10,14‐tetramethylpentadecane), a widespread isoprenoid hydrocarbon, has been studied in rats after a single per os administration of 3 H‐labeled pristane. The balance study showed an extensive fecal excretion (66%) mainly as unchanged hydrocarbon, whereas about 14% of ingested pristane was excreted in urine as pristane metabolites and tritiated water. After one wk, 8.3% of the ingested 3 H still was stored in the carcass, and radioactive distribution in tissues and organs showed a preferential incorporation into adipose tissue and liver. Over 75% of the radioactivity stored in the carcass was associated with pristane metabolites and tritiated water. Tissue metabolites were characterized by thin layer chromatography, gas chromatography and mass spectrometric analyses. Four metabolites were identified: pristan‐1‐ol, pristane‐2‐ol, pristanic acid and 4,8,12‐trimethyltridecanoic acid. These demonstrate that this isoprenoid hydrocarbon undergoes subterminal hydroxylation or terminal oxidation followed by the classical β‐oxidation process. Incorporation of metabolites in phospholipids and more particularly in the phosphatidylserine fraction has been observed and is discussed.