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( 3 H)Forskolin‐ and ( 3 H)dihydroalprenolol‐binding sites and adenylate cyclase activity in heart of rats fed diets containing different oils
Author(s) -
Alam Syed Q.,
Ren YunFang,
Alam Bassima S.
Publication year - 1988
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535459
Subject(s) - dihydroalprenolol , forskolin , menhaden , adenylate kinase , cyclase , endocrinology , medicine , biochemistry , chemistry , enzyme , biology , fish oil , receptor , agonist , fish <actinopterygii> , fishery , partial agonist
The characteristics of the cardiac adenylate cyclase system were studied in rats fed diets containing fish oil (menhaden oil) and other oils. Adenylate cyclase activity generally was higher in cardiac homogenates and membranes of rats fed diet containing 10% menhaden oil than in the other oils. The increase in enzyme activity, especially in forskolin‐stimulated activity, was associated with an increase in the concentration of the [ 3 H]forskolin‐binding sites in cardiac membranes of rats fed menhaden oil. The β‐adrenergic receptor concentration was not significantly altered although the affinity for [ 3 H]dihydroalprenolol‐binding was lower in membranes of rats fed menhaden oil than those fed the other oils. ω‐3 fatty acids from menhaden oil were incorporated into the cardiac membrane phospholipids. The results suggest that the observed increase in myocardial adenylate cyclase activity of rats fed menhaden oil may be due to an increase in the number of the catalytic subunits of the enzyme or due to a greater availability of the forskolin‐binding sites.