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Metabolism of sterols by anaerobic Saccharomyces cerevisiae
Author(s) -
Sekula Bernard C.,
Nes William R.
Publication year - 1981
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535438
Subject(s) - desmosterol , delta , saccharomyces cerevisiae , chemistry , sterol , stereochemistry , double bond , metabolism , yeast , biochemistry , cholesterol , organic chemistry , engineering , aerospace engineering
Anaerobically grown Saccharomyces cerevisiae retained the ability to transfer a C 1 ‐group to the C‐24 position of a Δ 24(25) ‐sterol and to reduce the Δ 24(28) ‐bond of a 24‐methylenesterol. Both desmosterol and 24‐methylenecholesterol yielded 24β‐methylcholesterol. However, when the substituent at C‐24 was enlarged to a 24‐ethylidene group (fucosterol), reduction of the Δ 24(28) ‐bond did not occur. In no cases was a Δ 7 a Δ 22 ‐bond introduced. Because the Δ 24(28) ‐bond was, reduced in the absence of the Δ 22 ‐bond the Δ 22 is not an obligatory requirement for reduction.