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Species differences in lipid peroxide levels in lung tissue and investigation of their determining factors
Author(s) -
Arakawa Kenji,
Sagai Masaru
Publication year - 1986
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535410
Subject(s) - npsh , polyunsaturated fatty acid , chemistry , thiobarbituric acid , lipid peroxidation , phospholipid , biochemistry , fatty acid , medicine , endocrinology , antioxidant , biology , enzyme , glutathione , membrane
Abstract Marked species differences in thiobarbituric acid reactant value (TBA value) in normal lung tissue of five species of animals were found. The order of the values was mouse > hamster > rat > guinea pig > rabbit, and the value for mice was 3.6 times higher than that for rabbit. The vitamin E (VE) and nonprotein sulfhydryls (NPSH) contents in lungs varied widely among the five animal species. Species differences were also observed on polyunsaturated fatty acid composition in lung phospholipids. The peroxidizability index (PI), which shows the relative rate of peroxidation reaction, was calculated from the composition ratio and the reactivity of each polyunsaturated fatty acid, and the PI was found to be significantly correlated to the TBA value in lungs (r=0.853, p<0.001). The PI value was normalized by the contents of VE and/or NPSH. Finally, the log‐value of PI, normalized by the log values of the reciprocals of VE and NPSH, log(PI/VE·NPSH), showed the highest correlation coefficient (r=0.907, p<0.001). Normalization by the activities of antioxidative protective enzymes in lungs did not show any significant correlation against TBA value. These results suggest that TBA value as an index of lipid peroxides in the lungs of animals may be regulated mainly by the contents of VE and NPSH, the composition ratio and the reactivity of each polyunsaturated fatty acid in lung phospholipid fraction.