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The effect of developing solvents on lipid class quantification in chromarod thin layer chromatography/flame ionization detection
Author(s) -
Parrish C. C.,
Ackman R. G.
Publication year - 1983
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535398
Subject(s) - flame ionization detector , chemistry , ionization , chromatography , thin layer chromatography , gas chromatography , polarity (international relations) , solvent , analytical chemistry (journal) , polar , ketone , detector , organic chemistry , optics , physics , cell , ion , biochemistry , astronomy
Undeveloped lipids on Chromarods give peaks of variable shape and of variable area when scanned through the flame ionization detector (FID). With a brief exposure to developing solvents of suitable polarity, it is possible to produce sharp peaks of less variable area without appreciably moving peak axes. Longer developments or developments in more polar solvent systems result in broader‐based peaks of lower area. These observations, based on many analyses of a triglyceride and of a ketone, suggest that the FID response of lipids can be modified according to their distribution around or along Chromarods.