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Metabolism of platelet‐activating factor by blood platelets and plasma
Author(s) -
Alam Iftekhar,
Smith J. Bryan,
Silver Melvin J.
Publication year - 1983
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535393
Subject(s) - platelet , chemistry , platelet activating factor , metabolism , high performance liquid chromatography , clinical chemistry , biochemistry , medicine , chromatography
High performance liquid chromatography in combination with a radioactivity detector was used to study the metabolism of platelet‐activating factor (1‐0‐alkyl‐2‐acetyl‐ sn ‐glycero‐3‐phosphocholine) by washed platelets, platelet‐free plasma and platelet‐rich plasma obtained from rabbits and humans. Degradation of platelet‐activating factor in plasma was completely inhibited by diisopropylfluorophosphate and was partially inhibited by ethylenediamine tetraacetic acid. Washed platelets metabolized platelet‐activating factor not only to the 2‐lyso compound but also, by reacylation of this lyso intermediate, to an analogue of platelet‐activating factor probably containing a long‐chain acyl group at the sn ‐2 position. These transformations occurred, but to a lesser extent, in platelet‐rich plasma.