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Isolation and determination of cholesterol glucuronide in human liver
Author(s) -
Hara Atsushi,
Taketomi Tamotsu
Publication year - 1982
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535377
Subject(s) - chromatography , glucuronide , chemistry , thin layer chromatography , sephadex , ganglioside , silica gel , column chromatography , cholesterol , lipidology , gel permeation chromatography , clinical chemistry , human liver , biochemistry , metabolism , in vitro , enzyme , organic chemistry , polymer
Separation of the acidic lipid fraction from human liver led to the identification of cholesterol‐β‐glucuronide for the first time from this organ. Cholesterol glucuronide was purified by DEAE‐Sephadex column chromatography and preparative silica gel thin‐layer chromatography. The content in normal human liver was about 33 nmol/g wet tissue. It must be emphasized that cholesterol glucuronide cannot be distinguished readily from ganglioside GM4 by thin‐layer chromatography.