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On the occurrence of α‐tocopherolquinone in rat tissue
Author(s) -
Bieri John G.,
Tolliver Teresa J.
Publication year - 1981
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535351
Subject(s) - saponification , chromatography , high performance liquid chromatography , lipidology , chemistry , thin layer chromatography , clinical chemistry , quantitative analysis (chemistry) , liver tissue , biochemistry , biology , endocrinology
The amount of α‐tocopherolquinone in rat liver has been reinvestigated comparing (a) a conventional procedure including saponification and thin layer chromatography followed by high peformance liquid chromatography (HPLC), with (b) the direct HPLC analysis of a total lipid extract. Recovery of added α‐tocopherolquinone was quantitative with both procedures. In contrast to a recent report of 124 nmol/g in rat liver, we found no more than 1–4 nmol/g by procedure a and less than 1 nmol/g by procedure b.