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High pressure liquid chromatographic separation of molecular species of phosphatidic acid dimethyl esters derived from phosphatidylcholine
Author(s) -
Hsieh John YK.,
Welch David K.,
Turcotte Joseph G.
Publication year - 1981
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535346
Subject(s) - phosphatidic acid , chemistry , degree of unsaturation , chromatography , phosphatidylcholine , methanol , phospholipid , acetonitrile , cardiolipin , trimethylsilyl , organic chemistry , biochemistry , membrane
A majority of the individual molecular species of phosphatidic acid dimethyl esters derived from multispecies egg yolk and soybean phosphatidylcholines have been separated by reverse‐phase high pressure liquid chromatography. Two Partisil‐10 ODS columns connected in tandem and the eluents acetonitrile or methanol/water (95∶5) were used for molecular species resolution, based on total fatty acyl carbon number and degree of unsaturation.