Premium
Ceramide structure of sphingomyelin from human milk fat globule membrane
Author(s) -
Bouhours JeanFrançois,
Bouhours Danièle
Publication year - 1981
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535339
Subject(s) - sphingomyelin , ceramide , lipidology , sphingosine , chemistry , globules of fat , chromatography , clinical chemistry , fatty acid , biochemistry , skimmed milk , phospholipase , sphingolipid , membrane , milk fat , food science , enzyme , apoptosis , receptor , linseed oil
Sphingomyelin was purified from human milk fat globule membrane and submitted to phospholipase C to yield ceramide. The structure of this ceramide was investigated by gas liquid chromatographic analyses of its components, fatty acids and sphingoid bases. The structure of the native ceramide was confirmed by direct‐inlet mass spectrometry. It was shown to contain a major base C 18 ‐sphingosine associated with a high proportion (60%) of C 20 , C 22 , C 24 and C 24∶1 nonhydroxylated fatty acids. As these very long‐chain fatty acids might be of nutritive importance, the concentration of sphingomyelin in human milk and its distribution in cream and skim milk were established.