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A novel bioaction of PAF: Induction of microbicidal activity in guinea pig bone marrow cells
Author(s) -
Hayashi Hidetoshi,
Kudo Ichiro,
Kato Toshiyuki,
Nozawa Ryushi,
Nojima Shoshichi,
Inoue Keizo
Publication year - 1988
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535276
Subject(s) - guinea pig , lipidology , clinical chemistry , bone marrow , chemistry , microbiology and biotechnology , biology , medicine , immunology , biochemistry
When guinea pig bone marrow cells were incubated in the presence of 10 −8 to 10 −6 M platelet activating factor (PAF) for 24 to 72 hr, microbicidal activity against Candida parapsilosis of cells was augmented. This augmentation was inhibited by PAF‐specific antagonists, CV6209 or FR900452. PAF‐specific binding sites with a high affinity were found on guinea pig bone marrow cells. Carrageenan or 2‐chloroadenosine, reagents known to be preferentially cytotoxic to macrophages, abolished the microbicidal activity of PAF‐treated bone marrow cells. Macrophages prepared from the peritoneal cavity, however, acquired no appreciable microbicidal action by treatment with PAF. These observations suggest that PAF may affect a class of guinea pig bone marrow cells through binding to receptors specific to PAF, resulting in activation and/or induction of differentiation of monocyte‐macrophage lineage cells.