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Location of double bonds in fatty acids of fish oil and rat testis lipids. Gas chromatography‐mass spectrometry of the oxazoline derivatives
Author(s) -
Yu Q. T.,
Liu B. N.,
Zhang J. Y.,
Huang Z. H.
Publication year - 1989
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535269
Subject(s) - lipidology , oxazoline , chemistry , mass spectrometry , clinical chemistry , fish <actinopterygii> , fish oil , gas chromatography–mass spectrometry , gas chromatography , chromatography , double bond , organic chemistry , biochemistry , catalysis , biology , fishery
A new approach to the derivatization and analysis of long chain polyunsaturated fatty acids is described. The method is based on the formation of 2‐alkenyl‐4,4‐dimethyloxazolines by condensation of the starting material with 2‐amino‐2‐methylpropanol. The derivatization method is rapid, efficient and specific with respect to the chain feature of the parent acids. Volatility, comparable with that of the corresponding simple esters, and improved gas chromatographic separation are achieved without difficulty. The derivatives exhibit clear and regular fragmentation patterns that allow easy discrimination of positional isomers and assignment of double bond location in the chain.