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Capillary gas chromatography and tandem mass spectrometry of paf‐acether and analogs: Absence of 1‐ O ‐alkyl‐2‐propionyl‐ sn ‐glycero‐3‐phosphocholine in human polymorphonuclear neutrophils
Author(s) -
Bossant M. J.,
Farinotti R.,
De Maack F.,
Mahuzier G.,
Benveniste J.,
Ninio E.
Publication year - 1989
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535248
Subject(s) - chemistry , lipidology , mass spectrometry , chromatography , alkyl , clinical chemistry , capillary action , phosphocholine , gas chromatography , capillary gas chromatography , tandem mass spectrometry , analytical chemistry (journal) , organic chemistry , biochemistry , phospholipid , membrane , phosphatidylcholine , materials science , composite material
Fast atom bombardment‐tandem mass spectrometry was used to identify molecular species of paf‐acether (paf) produced by human polymorphonuclear neutrophils. Using this biological material, normal phase high performance liquid chromatography was necessary prior to the fast atom bombardment‐tandem mass spectrometry step. Gas liquid chromatography/electron capture detection after hydrolysis with phospholipase C and conversion to heptafluorobutyrate derivatives was used to confirm the results. The results indicated the presence of mainly 1‐ O ‐hexadecyl/octadecyl‐2‐acetyl‐ sn ‐glycero‐3‐phosphocholine, acyl analogs of paf and only trace amounts of other alkyl analogs of paf. We did not detect the 2‐propionyl analog of paf. Moreover, supplementation of human polymorphonuclear neutrophils with sodium propionate did not result in formation of the 2‐propionyl analog of paf.