Premium
An improved procedure for bile acid extraction and purification and tissue distribution in the rat
Author(s) -
Locket Patricia L.,
Gallaher Daniel D.
Publication year - 1989
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535238
Subject(s) - extraction (chemistry) , chromatography , lipidology , solid phase extraction , clinical chemistry , distribution (mathematics) , bile acid , chemistry , biology , biochemistry , mathematics , mathematical analysis
Two bile acid extraction procedures were compared using endogenously radiolabeled tissues and feces. The method of Setchell et al. ( J. Lipid Res. 24 , 1085–1100, 1983) resulted in essential complete extraction, whereas that of Manes and Schneider ( J. Lipid Res. 6 , 376–377, 1971) gave recoveries between 56–82%. The time requirement for the method of Setchell et al. could be drastically reduced with no loss in extraction efficiency. Using extracts from endogenously labeled material, a purification procedure using C18 solid‐phase extraction cartridges was developed that recovers >90% of bile acids. The distribution of bile acids within the intestinal tract and liver of the rat was determined.