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Improved methods for the isolation and study of the C 18 , C 20 and C 22 monoethylenic fatty acid isomers of biological samples: Hg adducts, HPLC, AgNO 3 ‐TLC/FID, and ozonolysis
Author(s) -
Sebedio JL.,
Farquharson T. E.,
Ackman R. G.
Publication year - 1982
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535229
Subject(s) - degree of unsaturation , chromatography , chemistry , fractionation , adduct , high performance liquid chromatography , ozonolysis , thin layer chromatography , fatty acid , organic chemistry
The monoethylenic isomers of C 18 , C 20 and C 22 chain lengths of the depot fat of a nonhominid primate (cynomolgus monkeys, Macaca fascicularis ), fed a partially hydrogenated herring oil (IV=76.0) for 30 months, were examined by 2 different approaches. The first isolation method involved preparative gas liquid chromatography and argentation thin layer chromatography (TLC). The second sequence involved a chain‐length fractionation system based on the TLC of the methoxy‐bromomercuri quence involved a chain‐length fractionation system based on the TLC of the methoxy‐bromomercuri adducts of the total methyl esters to isolate groups of acids of common degrees of unsaturation, and then high performance liquid chromatography on a reverse‐phase column. In both cases, the monoethylenic isomer distribution was determined by ozonolysis in BF 3 /MeOH. Comparable results were obtained with the 2 methods. The second approach is recommended for small biological samples, especially for those containing a relatively high proportion of di‐ and other polyethylenic isomers which might interfere.