Premium
Lipid characteristics of RSV‐transformed Balb/c 3T3 cell lines with different spontaneous metastatic potentials
Author(s) -
Calorini Lido,
Fallani Anna,
Tombaccini Donatella,
Barletta Emanuela,
Mugnai Gabriele,
Di Renzo Maria F.,
Comoglio Paolo M.,
Ruggieri Salvatore
Publication year - 1989
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535205
Subject(s) - phosphatidylethanolamine , chemistry , phospholipid , phosphatidylcholine , biochemistry , rous sarcoma virus , arachidonic acid , polyunsaturated fatty acid , cell culture , lysophosphatidylcholine , cell , fatty acid , biology , gene , genetics , membrane , enzyme
Abstract To determine whether a metastatic phenotype may be corelated with a characteristic lipid pattern, we compared the lipid composition of low metastasizing Balb/c 3T3 cells transformed by the B77 strain of Rous sarcoma virus (B77‐3T3 cells) with that of a subclone isolated by growth in 0.6% agar, the B77‐AA6 cells, which exhibit a high capacity for spontaneous metastasis. B77‐3T3 cells revealed characteristics in their lipid composition common to other systems of transformed cells, i.e., an accumulation of ether‐linked lipids, a reduction of the more complex gangliosides, an increase of oleic acid (18∶1) and a decrease of arachidonic (20∶4) and C22 polyunsaturated fatty acids in phospholipids. High metastatic B77‐AA6 cells showed: a) an even more marked decrease of complex gangliosides; b) a more pronounced increase of 18∶1 and decrease of 20∶4 and 22 polyunsaturated fatty acids in certain phospholipid classes; and c) a higher percentage of alkyl‐acyl subfractions in both phosphatidylcholine and phosphatidylethanolamine than B77‐3T3 cells. Comparing the data for other systems of metastatic cells with those of lipid studies of spontaneously metastasizing B77‐AA6 cell system leads us to conclude that the metastatic phenotype is characterized by a change in ether‐linked lipids, rather than in fatty acids.