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Isolation and characterization of ganglioside 9‐ O ‐acetyl‐GD 3 from bovine buttermilk
Author(s) -
Bonafede Daniela M.,
Macala Lawrence J.,
CostantinePaton Martha,
Yu Robert K.
Publication year - 1989
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535204
Subject(s) - ganglioside , chemistry , chromatography , monoclonal antibody , ion exchange , antibody , biochemistry , ion , organic chemistry , immunology , biology
Bovine buttermilk contains a unique ganglioside, 9‐ O ‐acetyl‐GD 3 . In order to isolate large quantities of this ganglioside, a simplified isolation scheme which consists of several ion‐exchange and silica gel column chromatographic procedures was devised. The isolated 9‐ O ‐acetyl‐GD 3 was characterized on the basis of its thin‐layer chromatographic behavior, its immunoreactivity with a specific monoclonal antibody, JONES, and by conversion to authentic GD 3 by mild base treatment.