Isolation and characterization of ganglioside 9‐ O ‐acetyl‐GD 3  from bovine buttermilk | Zendy

Bonafede Daniela M. | Zendy; Macala Lawrence J. | Zendy; CostantinePaton Martha | Zendy; Yu Robert K. | Zendy

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Isolation and characterization of ganglioside 9‐ O ‐acetyl‐GD 3 from bovine buttermilk

Author(s) -

Bonafede Daniela M.,

Macala Lawrence J.,

CostantinePaton Martha,

Yu Robert K.

Publication year - 1989

Publication title -

lipids

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.601

H-Index - 120

eISSN - 1558-9307

pISSN - 0024-4201

DOI - 10.1007/bf02535204

Subject(s) - ganglioside , chemistry , chromatography , monoclonal antibody , ion exchange , antibody , biochemistry , ion , organic chemistry , immunology , biology

Bovine buttermilk contains a unique ganglioside, 9‐ O ‐acetyl‐GD 3 . In order to isolate large quantities of this ganglioside, a simplified isolation scheme which consists of several ion‐exchange and silica gel column chromatographic procedures was devised. The isolated 9‐ O ‐acetyl‐GD 3 was characterized on the basis of its thin‐layer chromatographic behavior, its immunoreactivity with a specific monoclonal antibody, JONES, and by conversion to authentic GD 3 by mild base treatment.

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