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Comparison of bile acid synthesis determined by isotope dilution versus fecal acidic sterol output in human subjects
Author(s) -
Duane William C.,
Holloway David E.,
Hutton Scot W.,
Corcoran Patricia J.,
Haas Nancy A.
Publication year - 1982
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535192
Subject(s) - isotope dilution , sterol , chemistry , feces , dilution , bile acid , chromatography , isotope , yield (engineering) , clinical chemistry , biochemistry , cholesterol , biology , mass spectrometry , microbiology and biotechnology , physics , materials science , quantum mechanics , metallurgy , thermodynamics
Fecal acidic sterol output has been found to be much lower than bile acid synthesis determined by isotope dilution ( J. Lipid Res. 17: 77, 1976). Because of this confusing discrepancy, we compared these 2 measurements done simultaneously on 13 occasions in 5 normal volunteers. In contrast to previous findings, bile acid synthesis by the Lindstedt isotope dilution method averaged 16.3% lower than synthesis simultaneously determined by fecal acidic sterol output (95% confidence limit for the difference −22.2 to −10.4%). When one‐sample determinations of bile acid pools were substituted for Lindstedt pools, bile acid synthesis by isotope dilution averaged 5.6% higher than synthesis by fecal acidic sterol output (95% confidence limits −4.9 to 16.1%). These data indicate that the 2 methods yield values in reasonably close agreement with one another. If anything, fecal acidic sterol outputs are slightly higher than synthesis by isotope dilution.