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Gas chromatographic assay of the diastereomeric composition of all‐rac‐α ‐tocopheryl acetate
Author(s) -
Scott Clifford G.,
Cohen Noal,
Riggio Patrick P.,
Weber Giuseppe
Publication year - 1982
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535182
Subject(s) - diastereomer , chromatography , chemistry , elution , analytical chemistry (journal) , stereochemistry
It has been established by an evaluation of 6 production samples of all‐rac‐α ‐tocopheryl acetate that all 4 racemates (RRS/SSR, RRR/SSS, RSR/SRS and RSS/SRR) are consistently present in equimolar amounts (SD<0.3, RSD<1.2%). An analysis of variance indicated variance due to signal noise to be consistent within a sample run but to vary from day to day. Variance due to area measurement was greater for the first and last eluted racemates than for the second and third. Peak width and asymmetry were found to be extremely sensitive to sample loading and, even within acceptable limits for good quantitation, the distortion was sufficient to give the elution profile the appearance of a sample composed of 4 components in unequal proportions increasing according to the order of elution.