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Synthesis and lipase catalyzed hydrolysis of thiolesters of 2‐, 3‐ and 4‐methyl octanoic acids
Author(s) -
Sonnet Philip E.,
Baillargeon Mary Welch
Publication year - 1989
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535152
Subject(s) - candida rugosa , lipase , chemistry , hydrolysis , organic chemistry , titration , catalysis , aspergillus niger , reagent , triacylglycerol lipase , chromatography , biochemistry , enzyme
Abstract Racemic 2‐, 3‐ and 4‐methyloctanoic acids were synthesized. The 2‐mercaptoethanol S‐esters of these branched acids, and of octanoic acid, were exposed to lipases of the fungi Candida rugosa and Aspergillus niger . Reactions were followed spectrophotometrically using Ellman's Reagent. Branching at the 3‐position retards lipolysis more severely than at either the 2‐ or 4‐positions. Features of the assay method are discussed with reference to titrimetry.