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The quantitative analysis of plant sterols
Author(s) -
Knights B. A.
Publication year - 1982
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535104
Subject(s) - sterol , plant sterols , lipidology , chromatography , cholesterol , chemistry , quantitative analysis (chemistry) , clinical chemistry , gas chromatography , biochemistry
Two methods for the quantitative analysis of plant sterols have been described. In the first, cholesterol is used for control of recovery and gas liquid chromatography (GLC) analysis. When cholesterol is present in the sterol mixture, a radioactive standard (usually cholesterol or sitosterol) is used to control recovery, and coprosterol is used to monitor GLC. The methods are exemplified for nitrogenfixing root nodules and for chloroplasts, respectively.