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A modification of the thiobarbituric acid reaction
Author(s) -
Bidder T. George,
Sipka Bona M.
Publication year - 1989
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535084
Subject(s) - lipidology , clinical chemistry , thiobarbituric acid , chemistry , chromatography , organic chemistry , biochemistry , antioxidant , lipid peroxidation
Abstract A simple modification of the thiobarbituric acid reaction, which removes nonspecific chromogenic reaction products, involves incubation at room temperature for 16–20 hr and a column chromatographic step. The chromogen‐concentrating capacity of the latter step is useful for determination of low concentrations of thiobarbituric acid‐reactive materials in biological preparations.