Premium
Essential fatty acid supplemented diet increases renal excretion of prostaglandin E 2 and water in essential fatty acid deficient rats
Author(s) -
Hansen Harald S.
Publication year - 1981
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535041
Subject(s) - weanling , medicine , endocrinology , urine , excretion , clinical chemistry , radioimmunoassay , essential fatty acid , prostaglandin , chemistry , fatty acid , polyunsaturated fatty acid , biochemistry
Abstract Weanling male rats were fed an essential fatty acid (EFA)‐deficient diet for 25 weeks and then switched to an EFA‐supplemented diet for 3 weeks. Control rats received the EFA‐supplemented diet for 25 weeks and then the EFA‐deficient diet for 3 weeks. Throughout the last 19 weeks, the rats were housed in metabolic cages once a week for a 24‐hr period. Urinary excretion of prostaglandin E 2 (PGE 2 ) was estimated by radioimmunoassay. Throughout a period of 12 weeks (weeks 13–24) water consumption increased ca. 60%, and urine output and PGE 2 excretion decreased ca. 45% and 70%, respectively, in the EFA‐deficient rats. Feeding EFA‐supplemented diet to the EFA‐deficient rats for 3 weeks decreased the water consumption and raised the urine output to that observed in the controls. However, the urine output was corrected within 1 day whereas the water consumption was not corrected until the second measurement 8 days after the dietary change. The PGE 2 excretion increased more than 9‐fold (from 18±8 ng/24 hr to 165±51 ng/24 hr) 1 day after EFA‐supplementation, followed by a decrease to 86±29 ng/24 hr over the following 2 weeks. On the basis of the present data, it is suggested that EFA deficiency in rats causes diminished PGE 2 excretion, which can be normalized by EFA supplementation. The normalization of the urine flow may, in part, be caused by the concomitant considerable increase in endogenous PGE 2 synthesis.