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Exchange of phosphatidylcholine between rabbit erythrocytes and plasma in vivo
Author(s) -
Smith Norman B.,
Rubinstein David
Publication year - 1981
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02535002
Subject(s) - phosphatidylcholine , in vivo , phosphatidylserine , chemistry , in vitro , clinical chemistry , acylation , biochemistry , chromatography , phospholipid , biology , membrane , microbiology and biotechnology , catalysis
Phosphatidylcholine exchange between rabbit erythrocytes and plasma was studied in vivo. The erythrocyte phosphatidylcholine was labeled by exchange in vivo with [ 32 P] phosphatidylcholine and in vitro by acylation with [ 3 H] 16∶0 and [ 14 C] 18∶2. The erythrocytes were then injected into rabbits and the loss of labeled phosphatidylcholine from the cells by exchange was followed. The rate constants for the exchange of [ 32 P]‐, [ 3 H] 16∶0‐, and [ 14 C] 18∶2‐phosphatidylcholine were .0131±.0010, .0093±.0014 and .0074±.0013 h −1 , and the exchange rates of the labels relative to that of [ 32 P] were 1.0, 0.71±.16, and 0.56±.14, respectively. These results confirm our earlier in vitro findings and represent the first in vivo demonstration of the dependency of the exchange rate of erythrocyte phosphatdylcholines on their metabolic prehistory.