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Rapid fractionation of human high density apolipoproteins by high performance liquid chromatography
Author(s) -
Polacek Denise,
Edelstein Celina,
Scanu Angelo M.
Publication year - 1981
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534999
Subject(s) - chromatography , fractionation , chemistry , high performance liquid chromatography , lipidology , human plasma , clinical chemistry , biochemistry
Abstract A simple and rapid fractionation procedure (30 min) has been developed for the isolation of the major apoproteins from human serum high density lipoproteins by molecular sieving in a high performance liquid chromatographic column. Apo A‐I, apoA‐II and the C peptides are quantitatively resolved up to a protein load of 3 mg. The technique has also been successfully applied to the final purification of A apoproteins which has been isolated by conventional chromatographic procedures and as a sensitive analytical tool for assessing apoprotein purity.