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Dry column method for the quantitative extraction and simultaneous class separation of lipids from muscle tissue
Author(s) -
Marmer William N.,
Maxwell Robert J.
Publication year - 1981
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534964
Subject(s) - chromatography , elution , chemistry , dichloromethane , chloroform , methanol , anhydrous , solvent , extraction (chemistry) , biochemistry , organic chemistry
Abstract A method for lipid isolation is presented that is alternative to the traditional chloroform/methanol extraction methods. This new method allows lipid isolation by solvent elution of a dry column composed of a tissue sample, anhydrous sodium sulfate, and Celite 545 diatomaceous earth groud together. To isolate total lipids, the dry column is eluted with a mixture of dichloromethane/methanol (90∶10, v/v). Alternatively, the lipids may be isolated and simultaneously separated into neutral and polar fractions by a sequential elution procedure; neutral lipids free of polar lipids are eluted first with dichloromethane, followed by elution of polar lipids with the dichloromethane/methanol (90∶10) mixture. The two dry column methods‐isocratic or sequential elution‐were compared with the traditional chloroform/methanol methods by gravimetric, thin layer chromatographic and phosphorus analyses.