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Effect of 2‐hexadecynoic acid on cultured 7288C hepatoma cells
Author(s) -
Upreti Girish C.,
Matocha Martha,
Wood Randall
Publication year - 1981
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534955
Subject(s) - acylation , metabolism , lipid metabolism , biochemistry , lipidology , fatty acid , chemistry , clinical chemistry , triglyceride , fatty acid synthesis , cell growth , dna synthesis , rna , dna , cholesterol , gene , catalysis
The effects of 2‐hexadecynoic acid on the growth and lipid metabolism of cultured 7288 (HTC) cells have been evaluated. Growth was inhibited by the acetylenic acid: the LD 50 was 35–85 μM as determined by two methods at low and high cell densities. Reduced growth did not result from damaged plasma membranes as determined by α‐amino isobutyrate leakage. DNA synthesis was unaffected by the acetylenic acid and the effect on RNA and protein synthesis appeared to be secondary to the effects on lipid metabolism. The 2‐hexadecynoic acid inhibited lipid metabolism of the HTC cells at least at two levels. Data from both mass studies and radioactive acetate distributions in cellular and media lipids indicated that fatty acid elongation and acylation, especially triglyceride synthesis, were inhibited.