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Purification of phospholipids by preparative high pressure liquid chromatography
Author(s) -
Geurts van Kessel W. S. M.,
Tieman M.,
Demel R. A.
Publication year - 1981
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534922
Subject(s) - chromatography , lipidology , chemistry , clinical chemistry , biochemistry
A method is described for the purification of a number of phospholipids by preparative high performance liquid chromatography (HPLC). Purification of digalactosyl‐diglyceride from spinach and egg phosphatidylcholine, 1,2‐dioleoyl‐ sn ‐glycero‐3‐phosphocholine and 1,2‐dioleoyl‐ sn ‐glycero‐3‐phosphoethanolamine from its reaction mixture have been resolved. The lipid separation is performed on a polygosil column and the individual compounds are monitored directly by refractive index detection. Chloroform/methanol mixtures are used as eluent systems, providing a wide polarity range to separate the classes of lipids. The developed equipment can be used for columns between 10 and 50 cm long and 4 and 50 mm inner diameter. The flow rate could be varied between 1 and 100 ml/min and applied pressures between 10 and 450 bars.