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Measurement of free and bound malondialdehyde in vitamin E‐deficient and‐supplemented rat liver tissues
Author(s) -
Lee HyeSung,
Saari Csallany A.
Publication year - 1987
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534861
Subject(s) - malondialdehyde , lipidology , clinical chemistry , vitamin e , chemistry , neurochemistry , biochemistry , medicine , biology , oxidative stress , antioxidant , neurology , neuroscience
The quantity of free malondialdehyde (MDA) in liver tissues of rats fed vitamin E‐deficient or‐supplemented diets for 43 wk was measured by a newly developed high performance liquid chromatographic (HPLC) method. Bound MDA was quantified by the same HPLC method after alkaline hydrolysis of tissue homogenates. Tissues from vitamin E‐deficient animals showed levels of free MDA about 15 times higher but levels of bound MDA less than 2 times higher than the vitamin E‐supplemented animals. Free MDA is the major form in vitamin E‐deficient tissues, but bound MDA is predominant in vitamin E‐supplemented tissues. Conventional thiobarbituric acid (TBA) test results revealed that the content of TBA‐reactive substances expressed in MDA equivalents was much higher than the actual free MDA levels in all groups. Results indicate that free MDA level measured by HPLC is a more sensitive index than the TBA value for lipid peroxidation. Some other TBA‐reactive substances seem to exist in liver tissue regardless of the dietary treatment.