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Complete separation of phospholipids from human heart combining two HPLC methods
Author(s) -
Juanéda Pierre,
Rocquelin Gérard
Publication year - 1986
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534829
Subject(s) - cardiolipins , chromatography , high performance liquid chromatography , chemistry , sphingomyelin , phospholipid , lipidology , human heart , clinical chemistry , biochemistry , cardiolipin , cholesterol , membrane , medicine , cardiology
The separation of phospholipid classes from human heart was achieved in two steps by high performance liquid chromatography (HPLC) using a silica column with an ultraviolet spectromonitor at 206 nm. A complete partitioning of phosphatidylcholines (PC), phosphatidylethanolamines (PE), phosphatidylinositols (PI), phosphatidylserines (PS), cardiolipins (CL), lysophosphatidylcholines (LPC) and sphingomyelins (Sph) was obtained for further analysis.