Complete separation of phospholipids from human heart combining two HPLC methods | Zendy

Juanéda Pierre | Zendy; Rocquelin Gérard | Zendy

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Complete separation of phospholipids from human heart combining two HPLC methods

Author(s) -

Juanéda Pierre,

Rocquelin Gérard

Publication year - 1986

Publication title -

lipids

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.601

H-Index - 120

eISSN - 1558-9307

pISSN - 0024-4201

DOI - 10.1007/bf02534829

Subject(s) - cardiolipins , chromatography , high performance liquid chromatography , chemistry , sphingomyelin , phospholipid , lipidology , human heart , clinical chemistry , biochemistry , cardiolipin , cholesterol , membrane , medicine , cardiology

The separation of phospholipid classes from human heart was achieved in two steps by high performance liquid chromatography (HPLC) using a silica column with an ultraviolet spectromonitor at 206 nm. A complete partitioning of phosphatidylcholines (PC), phosphatidylethanolamines (PE), phosphatidylinositols (PI), phosphatidylserines (PS), cardiolipins (CL), lysophosphatidylcholines (LPC) and sphingomyelins (Sph) was obtained for further analysis.

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