Premium
Separation of isomeric lysophospholipids by reverse phase HPLC
Author(s) -
Creer Michael H.,
Gross Richard W.
Publication year - 1985
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534778
Subject(s) - lipidology , chromatography , chemistry , phase (matter) , high performance liquid chromatography , clinical chemistry , organic chemistry , biochemistry
A reverse‐phase high performance liquid chromatography (HPLC) method was developed which resolved isomers of lysophosphatidylcholine (LPC) differing in the location of the aliphatic chain ( sn ‐1 or sn ‐2 position) and the position (Δ 6 or Δ 9 ) or geometric configuration ( cis or trans ) of the olefin group in monounsaturated species. LPC isomers containing an acyl substituent at the sn ‐2 position eluted before their 1‐acyl‐ sn ‐glycero‐3‐phosphocholine (1‐acyl LPC) counterparts. The retention times of both the sn ‐1 and sn ‐2 isomers of monounsaturated species increased in the order Δ 9 ‐ cis < Δ 9 ‐ trans < Δ 6 ‐ cis . The integrated ultraviolet absorbance (203 nm) in binary mixtures of the Δ 9 ‐ cis and Δ 6 ‐ cis 2‐acyl lysophospholipid isomers correlated with the lipid phosphorus content of corresponding column eluates ( r ‐0.994). Thus, the present method will facilitate synthesis of isomerically pure diradylphospholipids by providing homogeneous lysophospholipid precursors and help simplify the quantitative analysis of unsaturated lysophospholipid species.