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A modified radiometric assay for 3‐hydroxy‐3‐methylglutaryl coenzyme a reductase
Author(s) -
Georgopapadakou Nafsika H.,
Dix Barbara A.
Publication year - 1984
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534735
Subject(s) - reductase , coenzyme a , chemistry , biochemistry , chromatography , hydroxymethylglutaryl coa reductase , hmg coa reductase , radiometric dating , enzyme , biology , paleontology
A radiometric assay for measuring the activity of 3‐hydroxy‐3‐methylglutaryl‐coenzyme A (HMG‐CoA) reductase is described. The assay is based on the separation of the mevalonate product from HMG‐CoA by high‐voltage electrophoresis. This method is more sensitive and more specific than the NADPH‐based spectrophotometric assay, and less tedious than available radiometric assays. It has been used to measure HMG‐CoA reductase activity in crude extracts of Saccharomyces cerevisiae and in human skin fibroblasts.