Squalene isolation by HPLC and quantitative comparison by HPLC and GLC | Zendy

Sulpice J. C. | Zendy; Férézou J. | Zendy

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Squalene isolation by HPLC and quantitative comparison by HPLC and GLC

Author(s) -

Sulpice J. C.,

Férézou J.

Publication year - 1984

Publication title -

lipids

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.601

H-Index - 120

eISSN - 1558-9307

pISSN - 0024-4201

DOI - 10.1007/bf02534723

Subject(s) - squalene , chromatography , unsaponifiable , chemistry , high performance liquid chromatography , hexane , elution , fraction (chemistry) , silica gel , organic chemistry

A new procedure is described for isolating and measuring squalene in plasma and in several organs of the rat. The unsaponifiable material was fractionated by normal phase HPLC on a silica gel column using a mobile phase consisting of hexane/propanol‐2/water. The eluate was monitored at 215 nm. The squalene in the hydrocarbon fraction thus collected was than quantified on an analytical column eluted with hexane. Squalene concentrations ranging from 3 to 200 μg per ml of plasma or per g of fresh tissue were accurately measured. The results obtained agree with those of the squalene assays carried out by gas chromatography on a packed or capillary column.

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