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Asymmetric distribution of decanoate in triacylglycerol synthesized in vitro by mammary glands of lactating mice
Author(s) -
Rao G. Ananda,
Abraham S.
Publication year - 1983
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534711
Subject(s) - lipidology , clinical chemistry , in vitro , lactation , distribution (mathematics) , chemistry , mammary gland , endocrinology , medicine , milk fat , biochemistry , biology , food science , pregnancy , mathematics , genetics , mathematical analysis , cancer , breast cancer , linseed oil
Slices, prepared from the mammary glands of lactating mice, were incubated with either [1‐ 14 C]acetate, [U‐ 14 C]glucose, or [1‐ 14 C]decanoate. From all 3 substrates, radioactivity in the synthesized lipids was found mainly in triacylglycerols (TG). When acetate or glucose served as substrate, decanoate (C 10 ) accounted for 24% of the fatty acids in TG. Hydrolysis of the TG by pancreatic lipase yielded [ 14 C] fatty acids which had relatively more C 10 (38%) than did either of the other hydrolysis products mono‐ or diacylglycerol (14–17%). However, when TG produced by slices from C 10 were hydrolyzed, the acid was found to be esterified equally at the C‐1, C‐2 and C‐3 of glycerol. Thus, when fatty acids are synthesized de novo and are converted to TG by gland slices, C 10 is predominantly located in the C‐3 position, a finding in accord with the situation in milk TG, although such preferential incorporation does not occur when the free acid is presented to the tissue slices.