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Analysis of conjugated bile acids by high performance liquid chromatography and mass spectrometry
Author(s) -
Mingrone G.,
Greco A. V.,
Boniforti L.,
Passi S.
Publication year - 1983
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534697
Subject(s) - chromatography , chemistry , mass spectrometry , high performance liquid chromatography , bile acid , biochemistry
Because of the known advantages of coupling high performance liquid chromatography with mass spectrometry (HPLC‐MS) in biological fluids, studies on the reversed‐phase HPLC‐MS system for direct analysis of conjugated bile acids in human bile samples are described. Ten samples of gallbladder bile of apparently healthy subjects were examined. The amounts of each tauro‐ and glycoconjugated bile acid as trifluoracetate were determined by mass fragmentography. Quantitation of at least 1 ng of each bile acid was possible.