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Lipid and fatty acid characterization and metabolism in the sea anemone Phymactis clematis (Dana)
Author(s) -
Pollero Ricardo J.
Publication year - 1983
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534684
Subject(s) - clematis , lipidology , linolenate , glyceride , fatty acid , biochemistry , diacylglycerol kinase , biology , phospholipid , clinical chemistry , sea anemone , metabolism , lipid metabolism , chemistry , botany , enzyme , medicine , protein kinase c , herb , membrane , medicinal herbs , traditional medicine
Neutral lipid, phospholipids and fatty acids of the sea anemone Phymactis clematis from the south‐west Atlantic were characterized and quantified in spring and autumn. Neutral lipids predominated over phospholipids in both seasons. Triacylglycerol and diacylglycerol ethers were the major lipids. In spring, an increase of esterified sterols was noted. The major fatty acids found were 22∶5ω3, 20∶5ω3 and 16∶0. The sea anemones were also incubated in vivo with either [1‐ 14 C]linoleate or [1‐ 14 C] α‐linolenate for 2 hr. Isotope incorporation into lipids and their transformations into higher fatty acids were examined. Both precursors were incorporated into the lipids, mainly in triacylglycerols and mono‐acylglycerols, while α‐linolenate was also incorporated into phospholipids. The radioactive linoleate was elongated to 20∶2, 22∶2 and 24∶2 fatty acids, but not desaturated to 18∶3ω6. α‐Linolenate was desaturated by Δ6 desaturase to 18∶4ω3. The specificity of Δ6‐desaturase is discussed.