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Phospholipids of Rhizobium meliloti and Agrobacterium tumefaciens : Lack of effect of Ti plasmid
Author(s) -
Thompson Emily A.,
Kaufman Allan E.,
Johnston Norah C.,
Goldfine Howard
Publication year - 1983
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534669
Subject(s) - agrobacterium tumefaciens , phosphatidylethanolamine , phosphatidylglycerol , plasmid , rhizobium , phosphatidylcholine , phospholipid , cardiolipin , biology , rhizobiaceae , ti plasmid , transformation (genetics) , bacteria , microbiology and biotechnology , biochemistry , botany , gene , symbiosis , genetics , membrane
We have studied the phospholipid composition of Rhizobium meliloti strains which do or do not contain the large, tumor‐inducing (Ti) plasmid of Agrobacterium tumefaciens . The major phospholipids of stationary phase cells were phosphatidylethanolamine (PE) (22%), phosphatidyl‐N‐methylethanolamine (22%), phosphatidylcholine (PC) (27%), phosphatidylglycerol (11.4%), and cardiolipin (11%); as average percent of lipid phosphorus. Phosphatidyl‐N,N‐dimethylethanolamine (3.7%) was also present. The proportions of PE were higher, and PC lower, in logarithmic phase cells. No significant differences were seen in the proportions of phospholipids in strains with or without the Ti plasmid. Qualitative examination of the phospholipids of A. tumefaciens with or without the Ti plasmid similarly revealed no differences.