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The distribution of cholesterol and phospholipid composition in submitochondrial membranes from bovine adrenal cortex: Fundamental studies of steroidogenic mitochondria
Author(s) -
Cheng Behling,
Kimura Tokuji
Publication year - 1983
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534665
Subject(s) - submitochondrial particle , cardiolipin , phospholipid , membrane , inner membrane , mitochondrion , inner mitochondrial membrane , biochemistry , microsome , adrenal cortex , chemistry , bacterial outer membrane , biology , enzyme , escherichia coli , gene
The cholesterol contents and phospholipid compositions of mitochondria, microsomes and submitochondrial membranes from bovine adrenal cortex have been analyzed quantitatively. From our results, the following cholesterol contents were obtained: mitochondria, 6.2±0.9 mol %; microsomes, 18.4±2.8 mol %; mitochondrial inner membrane, 2.8±0.6 mol %; and mitochondrial outer membrane, 8.3±1.3 mol %. In addition, the phospholipid compositions of the mitochondrial inner and outer membranes were determined for the first time. Cardiolipin was found to be enriched in the inner membrane, whereas phosphatidylinositol was richer in the outer membrane. The general features of phospholipid compositions in the submitochondrial membranes resembled that of rat liver mitochondria.