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Effect of dietary fatty acids on Δ5 desaturase activity and biosynthesis of arachidonic acid in rat liver microsomes
Author(s) -
De Gómez Dumm I. N. T.,
De Alaniz M. J. T.,
Brenner R. R.
Publication year - 1983
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534636
Subject(s) - arachidonic acid , linoleic acid , palmitic acid , microsome , clinical chemistry , chemistry , biochemistry , biosynthesis , cycloheximide , fatty acid , biology , enzyme , medicine , protein biosynthesis
The effect of different fatty acids supplemented to a fat‐free diet on the activity of Δ5 desaturase was studied. Fat‐free diet produces a reduction in the conversion of eicosa‐8,11,14‐trienoic acid to arachidonic acid. The addition of the cis ‐ω6 acids, linoleic, γ‐linolenic or arachidonic to the diet produces an increase of eicosatrienoic acid desaturation, shifting Δ5 desaturase activity towards the controls on a balanced diet. This reactivation is apparently produced by induction of enzyme biosynthesis since linoleate effect was suppressed by simultaneous cycloheximide injection. On the contrary, no changes in Δ5 desaturation activity were found when the diet was supplemented with palmitic or 9‐ trans ,12‐ trans ‐linoleic acid. The changes on the activity of Δ5 desaturase were compared with the fatty acid composition of plasma and liver microsomes.