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Growth of a sterol auxotroph derived from Saccharomyces cerevisiae on chemically synthesized derivatives of cholesterol possessing side‐chain modifications
Author(s) -
Rodriguez R. J.,
Arunachalam T. A.,
Parks L. W.,
Caspi E.
Publication year - 1983
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534634
Subject(s) - sterol , auxotrophy , bromine , saccharomyces cerevisiae , cholesterol , side chain , yeast , chemistry , stereochemistry , biochemistry , organic chemistry , gene , mutant , polymer
A number of cholesterol derivatives were analyzed for their ability to satisfy bulk membrane and high‐specificity sparking requirements of a yeast sterol auxotroph (RD5‐R) (Rodriguez, R. J., Taylor, F. R., and Parks, L. W. [1982], Biochem. Biophys. Res. Commun. 106, 435–441). Substitution of hydrogen by bromine or iodine at C‐26 or substitution of C 26 ‐methyl by bromine enabled the resulting sterol to satisfy bulk or sparking functions. The presence of a side‐chain hydroxyl or keto group at C‐25 on a 26‐norcholesterol completely abolished the ability of cholesterol to satisfy either sterol requirement. Growth studies revealed that, while the oxygenated cholesterol derivatives were not growth‐supportive of RD5‐R, they were not growth‐inhibitory.