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Lipid deterioration: β‐Carotene destruction and oxygen evolution in a system containing lactoperoxidase, hydrogen peroxide and halides
Author(s) -
Kanner Joseph,
Kinsella John E.
Publication year - 1983
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534548
Subject(s) - lactoperoxidase , chemistry , halide , iodide , hydrogen peroxide , bromide , oxygen , inorganic chemistry , peroxide , photochemistry , peroxidase , organic chemistry , enzyme
A model system containing lactoperoxidase/H 2 O 2 /halide decomposed β‐carotene in a reaction greatly affected by the concentration of H 2 O 2 . The optimal concentrations of H 2 O 2 for activation of iodide and bromide were 2 mM and 10 μM, respectively. The oxidation of chloride by a lactoperoxidase, using β‐carotene destruction as a sensitive method to determine the activity of the enzyme, is reported herein. In the presence of optimal amounts of H 2 O 2 , the rate of β‐carotene destruction increases slowly until a critical concentration of the halides, followed by a rapid increase in the rate when halide concentrations were furthere increased. A lactoperoxidase/H 2 O 2 /iodide and/or bromide system generates oxygen in the presence of high H 2 O 2 and halide concentrations. β‐Carotene inhibited the evolution of oxygen. A possible mechanism of β‐carotene destruction and triplet unexcited oxygen evolution by a lactoperoxidase/H 2 O 2 /halide system are proposed.