Premium
Inhibition of the hormone‐sensitive lipase in adipose tissue by long‐chain fatty acyl coenzyme A
Author(s) -
Severson David L.,
Hurley Brenda
Publication year - 1984
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534504
Subject(s) - lipidology , adipose tissue , clinical chemistry , lipase , coenzyme a , hormone sensitive lipase , biochemistry , chemistry , enzyme , endocrinology , medicine , biology , reductase
The effects of free fatty acids and fatty acyl esters of coenzyme A and carnitine on the activity of a hormone‐sensitive lipase preparation made from pigeon adipose tissue were determined. Oleic acid (100 μM) resulted in a 40% inhibition of lipase activity A more potent inhibition of lipase activity was seen with long‐chain fatty acyl CoA compounds. The concentration required for half‐maximal inhibition with oleoyl CoA and palmitoyl CoA was 25–40 μM, whereas palmitoyl carnitine stimulated lipase activity. Activated lipase preparations (preincubated with Mg 2+ , ATP, cyclic AMP and protein kinase) were 4–6 times more sensitive to inhibition by oleoyl CoA than were nonactivated preparations. An increase in cellular levels of fatty acyl coenzyme A could, therefore, contribute to the feedback inhibition of lipolysis in adipose tissue.