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Effect of antioxidants on lipid peroxidation in iron‐loaded rats
Author(s) -
Dillard Cora J.,
Downey Jeanne E.,
Tappel Al. L.
Publication year - 1984
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534503
Subject(s) - lipidology , lipid peroxidation , clinical chemistry , chemistry , antioxidant , biochemistry , malondialdehyde , food science
Indirect evidence has suggested that lipid peroxidation is associated with iron overload in vivo. As a measure of lipid peroxidation, pentane expired in the breath of rats loaded with an accumulated dose of either 100 mg or 186–200 mg of iron injected intraperitoneally as iron dextran was measured over a 7 to 8 week period, and the effect on pentane production of feeding antioxidant‐supplemented diets was determined. By the seventh week of feeding the diets, rats fed 0.3% L‐ascorbic acid produced 17% less (P=0.03) pentane than did rats fed the basal antioxidant‐deficient diet, whereas rats fed 0.004% dl‐α‐tocopherol acetate produced 92% less (P<0.001). After being fed the basal diet for 7 weeks, iron‐loaded rats produced 76±9 pmol pentane/100 g body wt/min. When synthetic antioxidants were added to the diet at a concentration of 0.25%, the order of effectiveness in decreasing pentane production after 1 week was: N,N′‐diphenyl‐ p ‐phenylenediamine > ethoxyquin > butylated hydroxyanisole > butylated hydroxytoluene > propyl gallate ∼ no antioxidant. After removal of either ethoxyquin or N,N′‐diphenyl‐ p ‐phenylenediamine from the diets for 1 week, pentane production increased to a high level. The total amount of lipid soluble fluorophores in individual spleens of rats fed N,N′‐diphenyl‐ p ‐phenylenediamine, ethoxyquin, dl‐α‐tocopherol acetate, ascorbic acid and no antioxidant were correlated significantly with the corresponding total integrated amount of pentane produced by the individual rats over the 7 to 8 week period. This study has provided some of the most direct evidence to date that lipid peroxidation is associated with iron overload in vivo.