Premium
A rapid procedure for screening and isolating oxygenated free fatty acid metabolites from plants and natural waters
Author(s) -
Van Rogers A.,
Van Aller Robert T.,
Pessoney George F.,
Watkins Edward J.,
Leggett Harold G.
Publication year - 1984
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534461
Subject(s) - chromatography , extraction (chemistry) , high performance liquid chromatography , chemistry , fraction (chemistry) , solid phase extraction , fatty acid , aqueous two phase system , aqueous solution , organic chemistry
A rapid method of isolating a relatively pure fraction of oxygenated fatty acids from plants and natural waters is described. These metabolites were isolated from aqueous extracts using octadecylsilyl silica in a reverse‐phase batch extraction method. The extraction method, together with reverse‐phase analytical high pressure liquid chromatography (HPLC), was used to establish a routine screening method for the presence of these compounds in a variety of natural sources. A reverse‐phase preparative HPLC purification method is also described.