A rapid procedure for screening and isolating oxygenated free fatty acid metabolites from plants and natural waters | Zendy

Van Rogers A. | Zendy; Van Aller Robert T. | Zendy; Pessoney George F. | Zendy; Watkins Edward J. | Zendy; Leggett Harold G. | Zendy

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A rapid procedure for screening and isolating oxygenated free fatty acid metabolites from plants and natural waters

Author(s) -

Van Rogers A.,

Van Aller Robert T.,

Pessoney George F.,

Watkins Edward J.,

Leggett Harold G.

Publication year - 1984

Publication title -

lipids

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.601

H-Index - 120

eISSN - 1558-9307

pISSN - 0024-4201

DOI - 10.1007/bf02534461

Subject(s) - chromatography , extraction (chemistry) , high performance liquid chromatography , chemistry , fraction (chemistry) , solid phase extraction , fatty acid , aqueous two phase system , aqueous solution , organic chemistry

A rapid method of isolating a relatively pure fraction of oxygenated fatty acids from plants and natural waters is described. These metabolites were isolated from aqueous extracts using octadecylsilyl silica in a reverse‐phase batch extraction method. The extraction method, together with reverse‐phase analytical high pressure liquid chromatography (HPLC), was used to establish a routine screening method for the presence of these compounds in a variety of natural sources. A reverse‐phase preparative HPLC purification method is also described.

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