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Synthesis of the dipalmitoyl species of diacyl glycerophosphocholine by rabbit alveolar macrophages
Author(s) -
Nakagawa Yasuhito,
Waku Keizo
Publication year - 1986
Publication title -
lipids
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.601
H-Index - 120
eISSN - 1558-9307
pISSN - 0024-4201
DOI - 10.1007/bf02534438
Subject(s) - incubation , chemistry , phosphocholine , glycerol , biochemistry , pulmonary alveolus , clinical chemistry , chromatography , phosphatidylcholine , phospholipid , in vitro , membrane , macrophage
Abstract The distribution of radioactivity among the molecular species of diacyl glycerophosphocholine of rabbit alveolar macrophages was determined after incubation with [ 3 H]glycerol and 1‐[ 14 C]palmitoyl‐ sn ‐glycero‐3‐phosphocholine. The highest percentage of radioactivity of [ 3 H]glycerol was found in the dipalmitoyl species (35% of the total) followed by the 1‐palmitoyl‐2‐linoleoyl (23.6%) and 1‐stearoyl‐2‐linoleoyl plus 1‐palmitoyl‐2‐oleoyl species (19.7%) during the first 30 min incubation. The radioactivity of the dipalmitoyl species reached a maximum at 120 min incubation and decreased thereafter, although the radioactivities of other molecular species still increased. In contrast to the [ 3 H]glycerol labeling, only 4% of the total radioactivity in diacyl glycerophosphocholine derived from 1‐[ 14 C]palmitoyl glycerophosphocholine was found in the dipalmitoyl species; 80% of the radioactivity was located in the 1‐palmitoyl‐2‐arachidonoyl species at 10 min incubation. The present results indicate that the dipalmitoyl species of diacyl glycerophosphocholine are synthesized predominantly via a de novo pathway and not the deacylation‐reacylation pathway in rabbit alveolar macrophages.